Blue LED promotes the chemosensitivity of human hepatoma to Sorafenib by inducing DNA damage
نویسندگان
چکیده
Background: Phototherapies based on sunlight, infrared, ultraviolet, visible, and laser-based treatments present advantages like high curative effects, small invasion, negligible adverse reactions in cancer treatment. We aimed to explore the potential therapeutic effects of blue light emitting diode (LED) human hepatoma cells decipher underlying cellular molecular mechanisms. Methods: Wound healing transwell assays were employed probe inhibition invasion migration hepatocellular carcinoma presence LED. The sphere-forming test was used evaluate effect LED irradiation stem cell properties. Immunofluorescence western blotting detect changes γ-H2AX. Cell Counting Kit-8 assay, 5-ethynyl-2′-deoxyuridine staining, colony formation assay combined sorafenib proliferation inhibition. Results: demonstrated that could lead reduction along with increase apoptosis. Simultaneously, also markedly suppressed ability cells. Sphere analysis further revealed decreased stemness upon irradiation. Mechanistically, significantly promoted expression phosphorylation core histone protein H2AX (γ-H2AX), a sensitive marker DNA damage. In addition, we found treatment increased sensitivity sorafenib. Conclusion: Collectively, exhibited anti-tumor liver by inducing damage enhance chemosensitivity cells, which represents approach for
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ژورنال
عنوان ژورنال: Biocell
سال: 2023
ISSN: ['0327-9545', '1667-5746']
DOI: https://doi.org/10.32604/biocell.2023.029120